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mouse anti human stro 1  (R&D Systems)


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    R&D Systems mouse anti human stro 1
    Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and <t>stro-1,</t> and negative to cytokeratin-3 of hDPSCs
    Mouse Anti Human Stro 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab1038/Human+STRO-1+Antibody/pmc12990660-111-9-12
    Average 95 stars, based on 371 article reviews
    mouse anti human stro 1 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Salvianolic acid B functionalized injectable GelMA hydrogel for pulpitis in a vital pulp therapy: a dual anti-inflammatory property and enhanced reparative dentinogenesis activity material"

    Article Title: Salvianolic acid B functionalized injectable GelMA hydrogel for pulpitis in a vital pulp therapy: a dual anti-inflammatory property and enhanced reparative dentinogenesis activity material

    Journal: BMC Oral Health

    doi: 10.1186/s12903-026-07880-z

    Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs
    Figure Legend Snippet: Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

    Techniques Used: Light Microscopy, Staining, Immunofluorescence

    Related Articles

    Reverse Transcription:

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, Dako, 1:100 Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, Minneapolis, 5 μg/ml MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/ml Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Tex.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, 1:100 Dako, Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, 5 μg/mL Minneapolis, MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/mL Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Tex.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, Dako, 1:100 Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, Minneapolis, 5 μg/ml MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/ml Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Ill.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Polymerase Chain Reaction:

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, Dako, 1:100 Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, Minneapolis, 5 μg/ml MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/ml Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Tex.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, 1:100 Dako, Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, 5 μg/mL Minneapolis, MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/mL Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Tex.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, Dako, 1:100 Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, Minneapolis, 5 μg/ml MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/ml Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Ill.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Isolation:

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, Dako, 1:100 Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, Minneapolis, 5 μg/ml MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/ml Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Tex.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, 1:100 Dako, Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, 5 μg/mL Minneapolis, MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/mL Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Tex.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Article Title: Compositions and methods for cardiac tissue repair
    Article Snippet: .. Primary Antisera: Smooth muscle myosin heavy chain, SMMS-1, Dako, 1:100 Carpinteria, CA Von Willebrand Factor, F8/86, Dako 1:50 Human STRO-1, MAB1038, R and D Systems, Minneapolis, 5 μg/ml MN GATA 4, G-4, Santa Cruz Biotechnology, Santa Cruz, CA 1:100 Nkx 2.5, H-114, Santa Cruz 1:50 Cytokeratin (mixture), C2562, Sigma 1:100 Alpha smooth muscle actin, clone 1A4, A2547, Sigma 1:800 Sarcomeric actin, A2172, Sigma 1:500 SERCa2 ATPase, clone IID8, S1439, Sigma 1:400 Cardiac calsequestrin, C2491, Sigma 10 μg/ml Connexin 43, C6219, Sigma 1:400 Connexin 40, 36-4900, Zymed Laboratories (Invitrogen) 2 μg/ml Epicardin (TCF21), ab32981, Abcam 1:100 Myosin light chain phospho S20, ab2480, Abcam 1:100 Vimentin, E2944, Spring Bioscience, Freemont, CA 1:200 Secondary Antisera: Goat anti-mouse IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-mouse IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Goat anti-mouse IgM ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Goat anti-rabbit IgG ALEXA 488, Molecular Probes, Invitrogen 1:500 Donkey anti-goat IgG ALEXA 594, Molecular Probes, Invitrogen 1:800 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Total RNA was isolated from cell pellets using a commercial kit (RNAqueous, Ambion, Austin, Ill.). .. To avoid the possibility of contaminating DNA, the total RNA samples were treated with DNase prior to reverse transcription (TURBO DNase, Ambion).

    Marker:

    Article Title: A genomics approach in determining nanotopographical effects on MSC phenotype
    Article Snippet: .. Antibodies (with dilutions) used were directed to: osteogenic markers Phospho-Runx2 (Abgent, AP3559a) 1:500 total Runx2 (Santa Cruz Biotechnology, sc-10758) 1:500, stem cell marker STRO1 1:500 (R&D systems, MAB1038), adipocytic marker PParγ (Santa Cruz Biotechnology, sc-1984), chondrocytic marker SOX9 (Abcam, ab76997), housekeeping gene GapDH (Sigma, G8795); followed by the appropriate 1:4000 goat anti-rabbit, goat anti-mouse or donkey anti-goat IgG HRP-conjugates (Santa Cruz). .. Detection was performed by enhanced chemiluminescence (Immobilon Western, Millipore).

    Staining:

    Article Title: The relationship between cell proliferation and differentiation and mapping of putative dental pulp stem/progenitor cells during mouse molar development by chasing BrdU-labeling.
    Article Snippet: Human dental pulp contains adult stem cells.. Our recent study demonstrated the localization of putative dental pulp stem/progenitor cells in the rat developing molar by chasing 5-bromo-2’-deoxyuridine (BrdU)-labeling.. However, there are no available data on the localization of putative dental pulp stem/progenitor cells in the mouse molar.



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    Image Search Results


    Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

    Journal: BMC Oral Health

    Article Title: Salvianolic acid B functionalized injectable GelMA hydrogel for pulpitis in a vital pulp therapy: a dual anti-inflammatory property and enhanced reparative dentinogenesis activity material

    doi: 10.1186/s12903-026-07880-z

    Figure Lengend Snippet: Characterization of hDPSCs. A Light microscopy images of primary hDPSCs on 9th day. B Image of hDPSCs colony forming units at 14 days. C Alizarin red staining of mineralized nodules. D Oil red staining of adipogenic induction. E Alcian bale staining of chondrogenic induction. F Flow cytometric analysis of hDPSCs using multiple markers. hDPSCs were positive for CD73, CD90, and CD105, and negative for CD34 and CD45. G Positive immunofluorescence to nestin and stro-1, and negative to cytokeratin-3 of hDPSCs

    Article Snippet: Primary antibodies included rabbit anti-human nestin (Affinity Biosciences, China), mouse anti-human stro−1 (R&D systems, USA) and mouse anti-human cytokeratin (Abcam, USA).

    Techniques: Light Microscopy, Staining, Immunofluorescence